杨文超教授简历

个人简介

杨文超，男，理学博士，研究方向为农药化学生物学。国家高层次青年人才、香江计划入选者、湖北省杰出青年科学基金获得者。2009在华中师范大学化学学院获得有机化学博士学位，2005-2009年在美国肯塔基大学药学院进行博士联合培养，2010年在美国肯塔基大学药学院从事博士后研究。围绕绿色农药创制中“分子靶标与先导结构之间的相互作用关系”这一关键科学问题，长期从事农药的化学生物学以及绿色农药创制研究，在酶的催化机制研究、酶与抑制剂的相互作用，以及靶向酶的生物传感方面取得系统的研究成果。先后在Plant Physiology、J. Am. Chem. Soc.、ACS Nano、J. Agric. Food Chem.、Pest Manag. Sci.等国内外主流学术期刊上发表相关研究论文60余篇，获中国和国际发明专利授权10项。

工作经历

2023年4月——至今             贵州大学精细化工研究开发中心      教授

2017年7月——2023年3月      华中师范大学化学学院       教授

2012年7月——2017年6月      华中师范大学化学学院               副教授

2009年12月——2012年6月     华中师范大学化学学院       讲师

教育经历

2009年1月——2010年12月       美国肯塔基大学        博后

2005年1月——2009年10月      美国肯塔基大学        联合培养博士

2003年9月——2009年10月      华中师范大学          博士

1999年9月——2003年6月      华中师范大学          本科

学术兼职

2022年7月——2025年6月  《农药学学报》 青年编委/青年编委会副主任

2020年10月——2024年12月  Journal of Analysis and Test青年编委

教学工作

本科生课程：

1. 化学生物学（主讲）

2. 植保专业实验（参讲）

3. 农药学（参讲）

研究生课程：

1. 酶化学（主讲）

  研究方向

1. 农药化学生物学；

2. 生物分析化学；

3. 绿色农药创制。

科研项目

1.  国家重点研发计划(课题)：高选择性除草剂创制的分子基础与新品种开发（2021YFD1700103），2021/05-2025/12（主持）

2. 国家自然科学基金面上项目：基于小分子探针的线粒体呼吸链复合物II与复合物III的相互作用研究（21977036）

3.  国家自然科学基金面上项目：HPPD的催化反应机理及其与抑制剂相互作用的研究（21672079）

4. 国家自然科学基金面上项目：靶向细胞色素bc1复合物Qi位点的新型杀菌剂的设计与合成（21372094）

代表性论著

已在国内外发表学术论文70多篇，其中SCI论文60余篇；参编专著4部。

1. Fu, Y.X.; Guo, W.Y.; Wang, N.; Dai, Y.J.; Zhang, Z.Y; Sun, X.L.; Yang, W.C.*; Yang, G.F.Diagnosis of Bacterial Plant Diseases via a Nitroreductase-ActivatedFluorescent Sensor. Anal. Chem. 2022, 94, 17692−17699.

2. Fu, Y.X.; Zhang, Z.Y; Guo, W.Y.; Dai, Y.J.; Wang, Z.Y.; Yang, W.C.*; Yang, G.F.*In Vivo Fluorescent Screening for HPPD-Targeted Herbicide Discovery. Pest Management Science. 2022, 78, 4947–4955.（Front cover）

3. Fu, Y.X.;Liu, S.Y; Guo, W.Y.; Liu, G.Z.; Dong, J.; Nan, J.X.; Lin, H.Y.; Mei, L.C.; Yang, W.C.*; Yang, G.F.*In vivo diagnostics of abiotic plant stress responsesvia in situ real-time fluorescence imaging. Plant Physiology. 2022, 190, 196−201.

4. Guo, W.Y.; Li, R.R.; Liu, S.Y; Fu, Y.X.; Liu, G.Z.; Yang, W.C.*; Yang, G.F.Pyroglutamate Aminopeptidase I Promotes Hepatocellular Carcinoma via IL-6/STAT3 Activation as Revealed by a Specific Biosensor. Anal. Chem. 2021, 93, 13311−13318.

5. Lin, H.Y.; Chen X.; Dong, J.; Ye, Y.; Xiao, H.; Zhan, C.G.; Yang, W.C.*; Yang, G.F.* Rational Redesign of Enzyme via the Combination of QuantumMechanics/Molecular Mechanics, Molecular Dynamics, andStructural Biology Study. J. Am. Chem. Soc. 2021, 143, 15674−15687

6. X. Wang, H. Lin, J. Liu et al., The structure of 4-hydroxylphenylpyruvate dioxygenase complexed with 4-hydroxylphenylpyruvic acid reveals an unexpected inhibition mechanism, Chin. Chem. Lett., 2021,32, 1920−1924

7. Rajapaksha, A.A.; Fu, Y.X.; Guo, W.Y.; Liu, S.Y; Li, Z.W.; XiongC.Q.; Yang, W.C.*; Yang, G.F.Review on the recent progress in the development of fluorescent probes targeting enzymes. Methods and Applications in Fluorescence. 2021, 9, 032001.

8. Guo, W.Y.; Fu, Y.X.; Liu, S.Y; Mei, L.C.; Sun, Y.; Yin, J.; Yang, W.C.*; Yang, G.F.*Multienzyme-Targeted Fluorescent Probe as a Biosensing Platform for Broad Detection of Pesticide Residues. Anal. Chem. 2021, 93, 7079−7085.

9. Qu, R.Y.; Yang, J.F.; Chen, Q.; Niu, C.W.; Xi, Z; Yang, W.C.*; Yang, G.F.* Fragment-based discovery of flexible inhibitor targeting wild-type acetohydroxyacid synthase and P197L mutant. Pest. Manag. Sci.2020, 76, 3403–3412. 封面文章

10. Liu, S.Y.; Qu, R.Y.; Li, R.R; Yang, Y.C.; Sun, Y.; Yang, W.C.*; Yang, G.F.* An Activity-Based Fluorogenic Probe Enables Cellular and in Vivo Profiling of Carboxylesterase Isozymes. Anal. Chem. 2020, 92, 9205-9213.

11. Liu, S.Y.; Yan, A.M.; Guo, W. Y.; Fang, Y.Y.; Dong, Q.J.; Li, R.R; Ni, S.N.; Sun, Y.; Yang, W.C.*; Yang, G.F.* Human Neutrophil Elastase Activated Fluorescent Probe for Pulmonary Diseases Based on Fluorescence Resonance Energy Transfer Using CdSe/ZnS Quantum Dots. ACS Nano. 2020, 14, 4244-4254.

12. Liu, S.Y.; Xiong, H.; Li, R.R; Yang, W.C.*; Yang, G.F.* Activity-based Near-Infrared Fluorogenic Probe Enables In Vitro and In Vivo Profiling of Neutrophil Elastase. Anal. Chem. 2019, 91, 3877-3884.封面文章

13. He, B.; Dong, J.; Lin, H.Y.; Wang, M.Y.; Li, X.K.; Zheng, B.F.; Chen, Q.; Hao,G.F.; Yang, W.C.*; Yang, G.F.* Pyrazole−Isoindoline-1,3-dione Hybrid: A Promising Scaffold for 4 Hydroxyphenylpyruvate Dioxygenase Inhibitors.J. Agric. Food Chem. 2019, 67, 10844-10852.

14. Lin, H.Y.; Chen X.; Chen, J.N.; Wang, D.W.; Wu, F.X.; Lin, S.J.; Zhan, C.G.; Wu, J.W.; Yang, W.C.*; Yang, G.F.* Crystal Structure of 4-Hydroxyphenylpyruvate Dioxygenase in Complex with Substrate Reveals A New Starting Point for Inhibitor Discovery. Research. 2019, DOI:10.34133/2019/2602414. （国产高影响期刊）

15. Lin, H.Y.; Yang, J.F.; Wang, D.W.; Hao, G.F.; Dang, J.Q.; Wang, Y.X.; Yang, W.C.*; Wu, J.W.; Zhan, C.G.; Yang, G.F.* Molecular insights into the mechanism of 4-hydroxyphenylpyruvate dioxygenase inhibition: enzyme kinetics, X-ray crystallography and computational simulations. The FEBS J. 2019, 286, 975-990.

16. Ndikuryayo, F.; Wu, F.X.; Kang, W.M.; Yang, W. C.;* Yang, G. F.* Hydrophobicity-oriented drug design (HODD) of new human 4-hydroxyphenylpyruvate dioxygenase inhibitors. Eur. J. Med. Chem. 2019, 166, 22-31.

17. Liu, S.Y.; Xiong, H.; Yang, J.Q.; Yang, S.H.; Li, Y, Yang, W.C.*; Yang, G.F.* Discovery of Butyrylcholinesterase-Activated Near-Infrared Fluorogenic Probe for Live-Cell and In Vivo Imaging. ACS Sens.2018. 3, 2118−2128. 封面文章，并入选美国化学会ACS Editors' Choice.

18. .Li F.Y., Ndikuryayo,F.; Yang W. C.*, Wang H. M.*. Cholinesterases and Engineered Mutants for the.Detection of Organophosphorus Pesticide Residues.Sensors2018, 18, 4281.

19. Xiong, H.; Li, R.R; Liu, S.Y.; Wu, F.X.; Yang, W.C.*; Yang, G.F.* Discovery of Specific Nonpeptide Probe for Chymotrypsin via Molecular Docking-Based Virtual Screening and the Application. ACS Appl. Bio Mater. 2018, 1, 310−317.

20. Wu, S.; He, Y.J.; Qiua, X.X.; Yang, W.C.; Liu, W.C.; Li, X.H.; Li, Y.; Sheng, H.M.; Wang, R.X.; Yue, Z.Y.; Zhao, Y.X.*. Targeting the potent Beclin 1–UVRAG coiled-coil interaction with designed peptides enhances autophagy and endolysosomal trafficking. Proc. Natl. Acad. Sci. U.S.A.2018. 115. E5669-E5678.

21. Xiong, H.; Yang, J.Q.; Kang, W.M.; Wu, F.X.; Zuo, J.L.; Zhou, Z.Y.; Yang, W.C.*; Yang, G.F.* Fluorogenic and chromogenic detection of carboxypeptidase Y with anonpeptide-based small-molecule probe. Sensors and Actuators B.2018. 269. 127-134.

22. Yang, S.H.; Sun, Q.; Xiong, H.; Liu, S.Y.; Moosavi, B.; Yang, W.C. *; Yang, G.F.*Discovery of a butyrylcholinesterase-specific probe via a structure-based design strategy. Chem. Commun. 2017. 53, 3952-3955.

23. Ndikuryayo, F.; Moosavi, B.; Yang, W. C.*; Yang, G. F.* 4-Hydroxyphenylpyruvate Dioxygenase Inhibitors: From Chemical Biology to Agrochemicals. J. Agric. Food Chem., 2017, 65, 8523–8537.

24. 熊浩，杨文超, * 杨光富. * 胆碱酯酶荧光探针的研究进展. 中国科学：化学，2017，47，933-944.

25. Wu, L.; Yang, S.H.; Xiong, H.; Yang, J.Q.; Guo, J.; Yang, W.C. *; Yang, G.F. Nonpeptide-Based Small-Molecule Probe for Fluorogenic and Chromogenic Detection of Chymotrypsin. Anal. Chem. 2017, 89, 3687-3693.

26. Wang, D.W.; Lin, H.Y.; He, B.; Wu, F.X.; Chen, T.; Chen, Q.; Yang, W.C. *; Yang, G.F.* An Efficient One-Pot Synthesis of 2-(Aryloxyacetyl)cyclohexane-1,3-diones as Herbicidal 4-Hydroxyphenylpyruvate Dioxygenase Inhibitors..J. Agric. Food Chem. 2016, 64, 8986-8993.

27. Sun Q.; Yang S.H; Wu L.; Dong Q.J.; Yang, W. C.*; Yang, G. F.* Detection of intracellular selenol-containing molecules using a fluorescent probe with near-Zero background signal. Anal. Chem. 2016, 88, 6084-6091.

28. Sun Q.; Yang S.H; Wu L.; Yang, W. C.*; Yang, G. F.* A Highly Sensitive and Selective Fluorescent Probe for Thiophenol Designed via a Twist-Blockage Strategy. Anal. Chem. 2016, 88, 2266-2272.

29. Xu, Y.L.; Lin, H.Y.; Ruan, X.; Yang, S.G.; Hao, G.F.; Yang, W.C.*; Yang, G.F.*  Synthesis and bioevaluation of pyrazole-benzimidazolone hybrids as novel human 4-Hydroxyphenylpyruvate dioxygenase inhibitors. Eur. J. Med. Chem. 2015, 92, 427-438.

30. Wang, D.W.; Lin, H.Y.; Cao, R.J.; Ming, Z.Z.; Chen T.; Hao, G.F.; Yang, W.C.*; Yang, G.F.* Design, synthesis and herbicidal activity of novel quinazoline-2,4-diones as 4-hydroxyphenylpyruvate dioxygenase inhibitors. Pest. Manag. Sci.2015, 71 1122-1132.

31. Wang, D.W.; Lin, H.Y.; Cao, R.J.; Yang, S.G.; Chen, Q.; Hao, G.F.; Yang, W.C. *; Yang, G.F.* Synthesis and Herbicidal Evaluation of Triketone-Containing Quinazoline-2,4-diones.J. Agric. Food Chem. 2014, 62, 11786-11796.

32. Xu, Y.L.; Lin, H.Y.; Cao, R.J.; Ming, Z.Z.; Yang, W.C.*; Yang, G.F.* Pyrazolone-quinazolone hybrids: a novel class of human 4-hydroxyphenylpyruvate dioxygenase inhibitors.Bioorg. Med. Chem. 2014, 22, 5194-211.

33. Sun, Q.; Peng, D.Y.; Yang, S.G.; Zhu, X.L.; Yang, W.C.*; Yang, G.F.* Syntheses of coumarin-tacrine hybrids as dual-site acetylcholinesterase inhibitors and their activity against butylcholinesterase, Aβ aggregation, and β-secretase. Bioorg. Med. Chem. 2014, 22, 4784-91.

34. Li, J.; Zhang, C. F.; Yang, S. H.; Yang, W. C.*; Yang, G. F.* A coumarin-based fluorescent probe for selective and sensitive detection of thiophenols and its application. Anal. Chem.2014, 86, 3037-3042.

35. Sun Q.; Li J.; Liu W. N.; Dong Q. J.; Yang, W. C.*; Yang, G. F.* Non-peptide-based fluorogenic small-molecule probe for elastase. Anal. Chem. 2013, 85, 11304-11311.

36. Li, J.; Zhang, C. F.; Ming, Z. Z.; Yang, W. C.*; Yang, G. F.* Novel coumarin-based sensitive and selective fluorescent probes for biothiols in aqueous solution and in living cells. RSC Adv., 2013, 3, 26059-26065.

37. Li, J.; Zhang, C. F.; Ming, Z. Z.; Hao, G. F.; Yang, W. C.*; Yang, G. F.* Coumarin-based novel fluorescent zinc ion probe in aqueous solution. Tetrahedron2013, 69, 4743-4748.

38. Peng, D. Y.; Sun, Q.; Zhu, X.L.; Lin, H.Y.; Chen, Q.; Yang, W. C.*; Yang, G. F.* Design,  Synthesis, and Bioevaluation of Benzamides: Novel Acetylcholinesterase Inhibitors with Multi-functions on  Butylcholinesterase, Aβ Aggregation, and β-Secretase. Bioorg. Med. Chem. 2012, 20, 6739-6750.

39. Yang, W. C.; Li, H.; Wang, F.; Zhu, X.L.; Yang, G. F. * Rieske Iron–Sulfur Protein of the Cytochrome bc1 Complex: A Potential Target for Fungicide Discovery. Chembiochem2012, 134, 1542-1551.

40. Yang, W. C.; Li J.; Li J.; Chen Q.; Yang G. F.* Novel synthetic methods for N-cyano-1H-imidazole-4-carboxamides and their fungicidal activity. Bioorg. Med. Chem. Lett.2012, 22, 1455-1458.

41. Yang, W. C.; AbdulHameed M. D.; Hamza A.; Zhan C.G.* New inhibitor of 3-phosphoinositide dependent protein kinase-1 identified from virtual screening. Bioorg. Med. Chem. Lett. 2012, 15, 1629-1632.

发明专利

1. Zhao,Y.X.;  Wu, S.;Yang, W. C.; He, Y.J.;  Li, X.H.; Qiu, X.X. ；Hydrocarbon-stapled polypeptides for enhancement of endosome-lysosomal degradation，Patent #US 10618939，申请日期：2016年6月29日；   授权时间2018年3月14日

2. 杨光富，杨文超，杨庶候。用于检测丁酰胆碱酯酶的荧光探针及其制备方法和应用。申请号：201611121637.1，发明专利, 中国，已授权

奖励/荣誉情况

2014年 ，香江学者奖；

2021年 ，中国农药工业协会颁发的农药创新贡献一等奖：新型超高效除草剂喹草酮的创制与产业化，3/7；